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膽固醇-25-羥化酶(CH25H)重組蛋白
  • 品牌:KALANG/進口品牌
  • 產(chǎn)地:中國/美國
  • 貨號:kl-G357Hu01
  • 發(fā)布日期: 2016-03-09
  • 更新日期: 2024-12-23
產(chǎn)品詳請
產(chǎn)地 中國/美國
品牌 KALANG/進口品牌
貨號 kl-G357Hu01
保存條件 -20℃
英文名稱 Recombinant Cholesterol-25-Hydroxylase (CH25H)
CAS編號
保質(zhì)期 12個月個月

膽固醇-25-羥化酶(CH25H)重組蛋白

Recombinant Cholesterol-25-Hydroxylase (CH25H)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesHomo sapiens (Human)
Product No.
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass16.4kDa
Concentrationn/a
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1μg (determined by the LAL method)
ResiduesTrp142~Asn247 (Accession # O95992) with two N-terminal Tags, His-tag and T7-tag

FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

膽固醇-25-羥化酶(CH25H)重組蛋白SEQUENCES

The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFN

USAGE

Reconstitute in sterile ddH2O.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.

膽固醇-25-羥化酶(CH25H)重組蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

膽固醇-25-羥化酶(CH25H)重組蛋白About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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